History
The Department of Emergency Medicine has an established laboratory located on the Rhode Island Hospital campus in CORO West, Suite 4.303. This laboratory is staffed by investigators from the Department of Emergency Medicine and the Brown University Division of Engineering. Research activities are focused on Bio-Engineering and Biophotonics Projects which are translationally important to the care of Emergency Medicine patients.
Investigators and Collaborators
Gregory D. Jay, MD, PhD
Dr. Jay is a professor in the Department of Emergency Medicine and Division of Engineering at Brown University. He also serves as an adjunct professor in the Department of Biomedical Sciences at the University of Rhode Island. In addition to his academic activities, he brings almost 30 years of experience as an emergency medicine physician, and he is currently employed in that capacity by Brown Emergency Medicine within BPI, practicing at Rhode Island Hospital and Miriam Hospital in Providence, Rhode Island. Dr. Jay, who holds an MD, PhD (in experimental pathology) and double bachelor's degrees (in engineering and biochemistry) from State University of New York at Stony Brook, is a member of numerous professional societies and is a journal reviewer for several journals in the fields of engineering, emergency medicine, and several other medical specialties. He holds over a dozen issued patents and pending patent applications covering diverse technologies in pulsus paradoxus monitoring and the lubrication of mammalian joints. He has published 180+ articles in medical and scientific journals and is a recipient of support from NIH. He is co-editor of "Liquid Crystals: Frontiers in Biomedical Applications" (World Scientific Press, 2007). Dr. Jay is also a scientific co-founder of Lµbris, LLC based in Framingham, MA. This startup company is developing biotribological treatments for dry eyes, prevention of posttraumatic osteoarthritis, surgical adhesion prevention and other unmet medical needs. The technology central to the company’s growth after signing a licensing agreement with Novartis is the production of recombinant human PRG4 (lubricin) based on intellectual property and patents awarded to Dr. Jay and licensed to Lµbris.
Ling Zhang, MD
Dr. Ling Zhang is a senior research scientist in the Department of Emergency Medicine, Rhode Island Hospital. She received her MD from Henan Medical University in China. She worked as a research associate and was trained as a molecular and cellular scientist in the Department of Pharmacology and Toxicology at the Virginia Commonwealth University. Dr. Zhang was then appointed as a specialist to study immunology and toxicology. Prior to joining the Department of Emergency Medicine, she was the laboratory manager and senior research scientist investigating molecular mechanism(s) of inter-alpha inhibitor proteins in sepsis in the Department of Medicine at Rhode Island Hospital. Currently, Dr. Zhang is working in Dr. Jay's laboratory studying the molecular signaling pathway of Lubricin in mediating chondroprotection of diarthrodial joints. She has published 30 articles in medical and biological journals. Her primary expertise includes: cell and tissue culture, bone marrow culture, monoclonal antibody production, immunoblotting, ELISA, protein purification, molecular cloning, mutagenesis, siRNA, RNase protection assay, immunostaining, histology, flow cytometry, animal surgery, breeding and genotyping.
- Division of Biology and Medicine at Brown
- The Warren Alpert Medical School
- Center for Biomedical Engineering
What's Happening in the Lab?
Research Developments in the Laboratory
Recent Notable Publications
Qadri M, Jay GD, Zhang LX, Richendrfer H, Schmidt TA, Elsaid KA. Proteoglycan-4 regulates fibroblast to myofibroblast transition and expression of fibrotic genes in the synovium. Arthritis Res Ther. 2020 May 13;22(1):113. doi: 10.1186/s13075-020-02207-x. [PMID: 32404156] [PMCID: PMC7222325]
Bennett M, Chin A, Lee HJ, Morales Cestero E, Strazielle N, Ghersi-Egea JF, Threlkeld S, Schmidt T, Richendrfer H, Szmydynger-Chodobska J, Jay GD, Chodobski A. Proteoglycan 4 reduces neuroinflammation and protects the blood-brain barrier after traumatic brain injury. J Neurotrauma. 2020 Sep 17; doi: 10.1089/neu.2020.7229. [Epub ahead of print] [PMID: 32940130]
Flowers SA, Thommson KA, Ali L, Huang S, Mthembu Y, Regmi SC, Holgersson
J.Schmidt TA, Rolfson O, Bjorkman LI, Sundqvist M, Karlsson A, Jay GD, Eisler T, Krawetz R, Karlsson NG. (2020) Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking. Journal of Biological Chemistry. doi: 10.1074/jbc.RA120.012882. [PMID: 32928962] [PMCID: PMC7681006
Sojar SH, Quinn AM, Bortcosh WH, Decerbo PC, Chung E, LaVita CJ, Jay GD. Titration of Parameters in Shared Ventilation With a Portable Ventilator. Respir Care. 2020 Dec 9:respcare.08446. doi: 10.4187/respcare.08446. Epub ahead of print. [PMID: 3329862]
Menon NG, Goyal R, Lema C, Woods PS, Tanguay AP, Morin AA, Das N, Jay GD, Krawetz RJ, Dufour A, Shapiro LH, Redfern RL, Ghosh M, Schmidt TA. Proteoglycan 4 (PRG4) expression and function in dry eye associated inflammation. Exp Eye Res. 2021 May 25:108628. doi: 10.1016/j.exer.2021.108628. Epub ahead of print. [PMID: 34048779].
Suner S, Rayner J, Ozturan IU, Hogan G, Meehan CP, Chambers AB, Baird J, Jay GD. Prediction of anemia and estimation of hemoglobin concentration using a smartphone camera. PLoS One. 2021;16(7):e0253495. doi: 10.1371/journal.pone.0253495. eCollection 2021. PubMed PMID: 34260592; PubMed Central PMCID: PMC8279386.
Qadri M, Jay GD, Zhang LX, Schmidt TA, Totonchy J, Elsaid, KA. Proteoglycan-4 is an Essential Regulator of Synovial Macrophage Polarization and Inflammatory Macrophage Joint Infiltration. Arthritis Res Ther. 2021 Sep 14;23(1):241. doi: 10.1186/s13075-021-02621-9. PubMed PMID: 34521469; PubMed Central PMCID: PMC8439011
Duan C, Buerer L, Wang J, Kaplan S, Sabalewski, Jay GD, Monaghan, Arena AE, Fairbrother WG. Efficient Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Exhaled Breath. Journal of Molecular Diagnostics: JMD. 2021 Sep. DOI: 10.1016/j.jmoldx.2021.09.005. PMID: 34600137; PMCID: PMC8480135.
Elsayed S, Jay GD, Cabezas R, Quadri M, Schmidt TA, Elsaid K. Recombinant Human
Proteoglycan 4 Regulates Phagocytic Activation of Monocytes and Reduces IL-1β
Secretion by Urate Crystal Stimulated Gout PBMCs. Front. Immunol., 21 December 2021
doi:10.3389/fimmu.2021.771677.
Yang DS, Dickerson EE, Zhang LX, Richendrfer H, Karamchedu PN, Badger GJ, Schmidt TA, Fredericks AM, Elsaid KA, Jay GD. Quadruped Gait and Regulation of Apoptotic Factors in Tibiofemoral Joints Following Intra-articular rhPRG4 Injection in Prg4 Null Mice. Int J Mol Sci 8 April 2022. doi.org/10.3390/ijms23084245.
Khaled Elsaid, Tony R. Merriman, Leigh‐Ana Rossitto, Ru Liu‐Bryan, Jacob Karsh, Amanda Phipps‐Green, Gregory D. Jay, Sandy Elsayed, Marwa Qadri, Marin Miner, Murray Cadzow, Talia J. Dambruoso, Tannin Schmidt, Nicola Dalbeth, Ashika Chhana, Jennifer Höglund, Majid Ghassemian, Anaamika Campeau, Nancy Maltez, Niclas G. Karlsson, David J. Gonzalez, Robert Terkeltaub. Amplification of inflammation by lubricin deficiency implicated in incident, erosive gout independent of hyperuricemia. Arthritis & Rheumatology, 2022; DOI: 10.1002/art.42413. PubMed PMID: 36457235; NIHMSID:NIHMS1854456.
Das N, de Almeida LGN, Derakhshani A, Young D, Mehdinejadiani K, Salo P, Rezansoff A, Jay GD, Sommerhoff CP, Schmidt TA, Krawetz R, Dufour A. Tryptase β regulation of joint lubrication and inflammation via proteoglycan-4 in osteoarthritis. Nat Commun. 2023 Apr 6;14(1):1910. doi: 10.1038/s41467-023-37598-3. PMID: 37024468; PMCID: PMC10079686.
Grants:
Non-tribologic Bioactivity of Lubricin. National Institutes of Health National Institute of Arthritis and Musculoskeletal and Skin Diseases, 1R01AR067748. Principal Investigator. $2,190,726 awarded 5/10/16 – 4/30/2021.